|
|
|
Introduction
|
|
|
|
ZytoLight® products are designed for the identification of genetic aberrations, e.g. translocations, deletions, amplifications, and chromosomal aneuploidies by Fluorescence in situ Hybridization (FISH) in formalinfixed, paraffin-embedded tissue sections, cell samples, blood or bone marrow smears, and metaphase chromosome spreads.
|
|
|
Method Description
|
|
|
The ZytoLight® System uses direct labeled FISH probes (1), eliminating the need to detect the probes with fluorophore-coupled- antibodies. The probes are detected by fluorescence microscopy using appropriate filter sets (2). Due to an exciter filter (3), full-spectrum light, emitted by the microscope lamp (4), is reduced to light of a defined wavelength that specifically excites the fluorophore of the probe. This light is reflected onto the specimen by a dichroic mirror (5). The fluorophore emits light of longer wavelengths that passes the mirror. Finally, a barrier filter (6) reduces the emitted light to a defined wavelength that can be detected.
FISH analysis of paraffin-embedded tissues and metaphase spreads using the ZytoLight® System
Breast tissue section showing HER2 gene amplification (left). Detection of t(2;13) translocation in alveolar rhabdomyosarcomas as indicated by one red, one green and one fusion signal in each nuclei (middle). Normal male metaphase spread hybridized with CEN X/Y Dual Color Probe (right).
|
|
High Sensitivity and Specificity
|
|
ZytoLight® FISH probes are direct labeled using the unique ZytoLight® Direct Label System II providing improved signal intensity. All ZytoLight® single copy (SPEC) probes are processed by the unique ZytoVision® Repeat Subtraction Technique resulting in advanced specificity and less background. No further blocking of repetitive sequences is needed!
|
|
ZytoLight® SPEC and CEN Probes
|
|
ZytoLight® SPEC probes hybridize to specific, single copy DNA sequences of the human genome. ZytoLight® SPEC probes are available for the detection of a variety of chromosomal aberrations associated with tumors and genetic diseases.
ZytoLight® CEN Probes hybridize to highly repetitive human satellite DNA sequences located at the centromeric regions of chromosomes producing sharp, bright signals specific for each individual chromosome.
|
|
Find Your Probe
|
|
To find the appropriate FISH probe for your applications, please search our product list by selecting one of the chromosomes or an indication listed below. Subsequently, just click on the desired product for more information.
|
|
