Introduction

FlexISH® products are designed for identification of chromosomal aberrations on various specimens by Fluorescence in situ Hybridization (FISH). Using the FlexISH® products gives you the flexibility to choose  between a 1-day  (2 h hybridization) or a 2-day (overnight hybridization) protocol by adapting the hybridization time just according to your individual needs! 

FlexISH® brings Flexibility to Your FISH

  • FlexISH® maximizes your flexibility in terms of time and laboratory management. Hybridization time can be varied between 2 hours and overnight.
  • With a hybridization temperature of 37°C the FlexISH® protocol is fully compatible with routine workflows in pathology laboratories.
  • In a recent comparison study, overnight and short hybridization periods showed excellent correlation between the FISH results obtained with FlexISH®, ZytoLight®, and PathVysion ERBB2 probes1.
  • Short hybridization time does not negatively affect the performance, specimen quality or diagnostic result1.

 


Method Description & FlexISH® Fluorochromes F<em>lex</em>ISH <sup>®</sup> Method Description

The FlexISH® system uses direct labeled FISH probes. The probes are detected by fluorescence microscopy using appropriate filter sets. Due to an exciter filter, full-spectrum light, emitted by the microscope lamp, is reduced to light of a defined wavelength that specifically excites the fluorophore of the probe. This light is reflected onto the specimen by a dichroic mirror. The fluorophore emits light of longer wavelengths that passes the mirror. Finally, a barrier filter reduces the emitted light to a defined wavelength that can be detected.

Two factors that mainly influence FISH analyses:

Please refer to your local distributor for precise information regarding the suitable filter sets.

 

FlexISH® Kits – Convenient Solutions

All FlexISH® FISH probes can be combined with the:

 


References:
1. Brockhoff G, et al. (2016) Histopathology 69: 635-646.

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