Introduction
FlexISH® products are designed for identification of chromosomal aberrations on formalin-fixed, paraffin-embedded (FFPE) specimens by Fluorescence in situ Hybridization (FISH). Using the FlexISH® products gives you the flexibility to choose between a 1-day (2 h hybridization) or a 2-day (overnight hybridization) protocol by adapting the hybridization time just according to your individual needs!
FlexISH® brings Flexibility to your FISH
- FlexISH® maximizes your flexibility in terms of time and laboratory management.
- Hybridization time can be varied between 2 hours and overnight.
- With a hybridization temperature of 37°C the FlexISH® protocol is fully compatible with routine workflows in pathology laboratories.
Method Description & FlexISH® Fluorochromes
The FlexISH® system uses direct-labeled FISH probes. The probes are detected by fluorescence microscopy using appropriate filter sets. Due to an exciter filter, full-spectrum light, emitted by the microscope lamp, is reduced to light of a defined wavelength that specifically excites the fluorophore of the probe. This light is reflected onto the specimen by a dichroic mirror. The fluorophore emits light of longer wavelengths that passes the mirror. Finally, a barrier filter reduces the emitted light to a defined wavelength that can be detected.
Two factors that mainly influence FISH analyses:
- Fluorochromes of the FISH probes
- Appropriate filter sets
Please refer to your local distributor for precise information regarding the suitable filter sets.
FlexISH® Kits – Convenient Solutions
FlexISH® FISH probes are intended to be used with the:
- FlexISH®-Tissue Implementation Kit (Z-2182-5/-20) for FISH analyses on FFPE specimens