The ZytoFast® products are designed for outstandingly fast detection and discrimination of human pathogen viruses, e.g. HPV, EBV, CMV, and the determination of lymphocyte clonality by detecting Ig-κ and Ig-λ light chain RNA by Chromogenic in situ Hybridization (CISH) in formalin-fixed, paraffin-embedded tissue sections and cell samples. The signal intensity of ZytoFast® probes is increased even more when using the ZytoFast® PLUS Implementation Kits.
The ZytoFast® system uses oligonucleotide probes tagged with Biotin which are detected using enzyme-conjugated streptavidin targeting the tag. The enzymatic reaction of chromogenic substrates, e.g. BCIP/NBT or AEC, leads to the formation of strong color precipitates that can be visualized by light microscopy.
The ZytoFast® PLUS system uses Digoxigenin-labeled probes which are detected using primary antibodies. These antibodies are detected by polymerized enzyme-conjugated secondary antibodies. The enzymatic reaction of chromogenic substrates, e.g. NBT/BCIP, AEC or DAB, leads to the formation of strong color precipitates that can be visualized by light microscopy.
ZytoFast® - Flexibility that meets your needs
- All Biotin-labeled probes can be combined with a ZytoFast® CISH Implementation Kit
- All Digoxigenin-labeled probes can be combined with any of the ZytoFast® PLUS CISH Implementation Kits