The VisionArray HPV High Risk Chip 1.0 is intended to be used with the VisionArray Analysis Package for the qualitative detection and genotyping of PCR amplificates of 12 high-risk and 12 probably high risk Human Papilloma Virus (HPV) genotypes that have been produced with the help of the VisionArray HPV Primer Kit and the VisionArray Detection Kit.

The VisionArray FFPE DNA Extraction Kit is designed to be used for the isolation of genomic DNA from formalin-fixed, paraffin-embeded (FFPE) specimens. The professional use of this purification system optimized for FFPE samples results in highly purified nucleic acid samples. The purified DNA can be used directly for PCR with the VisionArray HPV Primer Kit, the VisionArray PreCise Taq DNA Polymerase, and the VisionArray Uracil-DNA (UDG) Glycosylase.

The VisionArray Cytology DNA Extraction Kit is designed to be used for the rapid isolation of genomic DNA from liquid-based cytology samples in order to achieve small-scale preparations of highly pure genomic DNA. The purified DNA can be used directly for PCR with the VisionArray HPV Primer Kit, the VisionArray PreCise Taq DNA Polymerase, and the VisionArray Uracil-DNA (UDG) Glycosylase.

The VisionArray HPV Primer Kit is intended to be used for amplifying HPV types including but not limited to those detected by the VisionArray HPV Chips. During PCR, specific sections of the L1 region of the Human Papilloma Virus (HPV) will be biotinylated. The hybridization and detection of the amplified sequences is subsequently performed using the VisionArray Detection Kit and the VisionArray HPV Chip.

The VisionArray PreCise Taq DNA Polymerase is a high quality heat stable Taq polymerase intended to be used for the specific amplification of a wide range of DNA fragments. The enzyme has hot-start capabilities, reduces false positives, amplifies low-level target sequences, and promotes amplification of a variety of templates. Further on, neccessary for succesful HPV genotyping is the use of the VisionArray HPV Primer Kit in combination with the VisionArray Uracil-DNA (UDG) Glycosylase. Hybridization and detection of the amplified sequences is performed subsequently using the VisionArray Detection Kit and the VisionArray HPV Chip.

The VisionArray Uracil-DNA (UDG) Glycosylase is an E. coli derived enzyme intended to be used to prevent carry-over contaminations from previous reactions. This is based on the principle of substituting dUTPs for dTTPs during PCR. Therefore the PCR amplificate can be "discriminated" from the initital DNA strand. Incubating the PCR mixture with the UDG enzyme at elevated temperatures under alkaline conditions (during the initial denaturation step) will remove contaminating U-DNA from the sample thereby avoiding false positive results.

The VisionArray Detection Kit is designed for the qualitative detection of specific DNA sequences produced with the VisionArray Primer Kit, the VisionArray PreCise Taq DNA Polymerase, the VisionArray Uracil-DNA (UDG) Glycosylase, and the corresponding VisionArray DNA Chip. The VisionArray Detection Kit contains all reagents that are required for the simple and fast hybridization and detection of PCR products on the VisionArray Chips.

The VisionArray Analysis Package stands for a simple visualization and a quick analysis of the VisionArray Chip data. The analysis of a chip and the report of the results can be achieved in just a few minutes.