ZytoLight® system uses direct-labeled FISH probes, which eliminate the need to detect the probes with fluorophore-coupled antibodies. ZytoLight® products are designed for the identification of genetic aberrations e.g. translocations, deletions, amplifications, and chromosomal aneuploidies by Fluorescence in situ Hybridization (FISH) in formalin-fixed, paraffin-embedded (FFPE) tissue sections or cytology specimens.


Method Description & ZytoLight® Fluorochromes Zyto<em>Light</em><sup>®</sup> Method Description

The ZytoLight® system uses direct-labeled FISH probes. The probes are detected by fluorescence microscopy using appropriate filter sets. Due to an exciter filter, full-spectrum light, emitted by the microscope lamp, is reduced to light of a defined wavelength that specifically excites the fluorophore of the probe. This light is reflected onto the specimen by a dichroic mirror. The fluorophore emits light of longer wavelengths that passes the mirror. Finally, a barrier filter reduces the emitted light to a defined wavelength that can be detected.

Two factors that mainly influence FISH analyses:

Please refer to your local distributor for precise information regarding the suitable filter sets.


ZytoLight® Kits – Convenient Solutions

For making FISH analysis reliable and user-friendly, ZytoLight® FISH probes are intended to be used with the appropriate Implementation Kit (annotated in the individual probe's instruction for use):

Both Implementation Kits include all necessary pretreatment solutions, wash buffers and DAPI/DuraTect-solution and a detailed protocol to perform successful FISH experiments.




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